Serology-Immunology

Hi all,

Really sry ppl. So pai sei leh. I made a big mistake in the TPHA test thing.

1. The test cells are sensitised cells that reacts specifically to the antibody produced in response to the causative agent. It is by default brown in color.

2. After everything is added in, the solution in the wells are mixed evenly by tapping gently.

3. How the particles settle after the incubation gives you the result. (so must take care not to agitate the plate during the incubation and even before you read and verify the results.)

4. Particles concentrated in the shape of a button in the centre of the well with a smooth round outer margin is read as non reactive.

5.Definite large ring with a rough multiform outer margin and peripheral agglutination is read as reactive.

6. Particles concentrated in the shape of a compact ring with a smooth round outer margin is read as a boderline case. This is reported as "possible biological false positive result". A repeat testing in 10 days along with a FTA-antibody assay would be suggested to clarify if the patient is positive or negative.



Hope that helps in clafying some doubts =)
Sincerely,
Yeng Ting