Microbiology--- CAMP TEST (week 19)

hey to all!!!

I'm posting for week 19!!! TIme passed super fast...

I saw ying ying's posts abt CAMP test but what i'm abt to post is a bit different from her.

Here i go...

For those who had been to micro lab, you should pretty well known with GPB/GPC/GNB/GNC.

Here i'm going to introduce you to ARCANOBACTERIUM HAEMOLYTICUM.

CHARACTERISTICS:

--- gram postive rods with pointed ends

---catalase negative

---small colonies, may be smooth, mucoid, white, dry

---B-haemolytic

---It will not grow on MacConkey agar

--- Facultative anaerobe

Certain organism such as Group B streptoccui able to produce a factor that acts synergistically with B-hemolytic of S.aureus subspec. aureus on sheep blood. The purpose of test to determine the organism's ability to produce and elaborate CAMP factor. The production of a-hemolytic(a-toxin) by clostridium perfrigen also demostrate the synergistic phenomenon with CAMP factor. If Phospholipase D is produced by organism, the CAMP reaction would be inhibited.

Medium:

TSA with 5% sheep blood agar (BAP)

Procedure:

1) preheat 35'C. Ensure plates are dry.

2) Using inoculating needle, streak B-lysin substrate organism in a straight and across centre of BAP

3) Streak QC stain ( about 2-3cm) perpendicular to S.aureus inoculum without touching the Staphylococcui

4) Incubate CO2 35'c incubator for at least 24 hours

**Note: the arrow head means +ve CAMP results
The curve head means -ve CAMP results



MY senior said that normally she will do another REVERSE CAMP test(RCT) to confirm the results from CAMP test.


PRCT-- production of a distinct crescent hemolysis at junction between streptoccus agalatiae streak and test organism.

NPRCT-- No constriction of band of hemolysis






**Note: Postive reaction

Limitation:

--> size of zone is depend on the amount of bacterial product and its diffuse rate through the medium

--> inocula should be sufficient to produce confluent growth. The extent and intensity of lysis depends on the size of the staphylocuccus inoculum. If the inocula is too light, reaction would be weak.

--> If the inocula are not perpendicular, production of arrow heads will not appear. False Negative occur.







ELaIne

SEE ya soon!!!